The Function of Spore Grafts in the Research of Botrytis cinerea

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The damage of tomatoes to Botrytis cinerea is very serious and has happened in all parts of the country. It is necessary to monitor and control the Botrytis cinerea in order to be able to produce protective tomatoes. Although there have been many studies at home and abroad for the epidemic-influencing factors of the disease, there is still a lack of systematic research on the spore dispersal dynamics of the pathogen. Apply the most advanced multi-point meteorological data collectors at home and abroad to record data such as air temperature, humidity and sunshine intensity. We will use a self-developed spore capture instrument to continuously monitor the dynamics of conidial sporulation in the air, and perform correlation analysis on the data obtained to determine the correlation between environmental factors and spore scattering dynamics in the air.

A multi-point meteorological data collector is placed in a solar greenhouse, and the air temperature and relative humidity data collected in the collector are directly input into the computer through a data transponder every 7 days for analysis and processing. At the same time, the outdoor air temperature, relative humidity, precipitation, and weather changes were recorded in the same manner. The spore trap captures spores in the air.

Through spore trapping instrument combined with relevant data of meteorological collector, it was found that, when the greenhouse is low temperature and high humidity, in fact, the vegetable farmer does not ventilate and reduce humidity in order to prevent freezing damage. Prior to the outbreak of gray mold in sunlight greenhouses in 2001, most of them were fine weather, and the air in the greenhouse was normal. The amount of spores of spores flying in the air increased. After the outbreak of the disease, the amount of spores of pathogenic bacteria scattered in the air due to high humidity in the greenhouse decreased. The spores of pathogenic bacteria in the solar greenhouse are generally spread through droplets that collect on the greenhouse plastic sheet when the relative humidity is increased, and can also be transmitted through artificial agricultural operations. Continuous rainy days are conducive to a large number of pathogenic bacteria producing spores. The amount of spores flying in the air increases, and the continuous rainy days cause the relative humidity of the indoor air in the shed to increase, and the spores easily adhere to the leaves of the plants, which is not conducive to spore scattering. The amount of spores scattered in the early period was significantly greater than that in the late period, and the days of overcast and rainy days were more than in the early period, indicating that the degree of influence on spore scattering was increased and the relative humidity of the air was greater than that of continuous rainy days.

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